Anti-PCNA antibody

• Supplier: Abcam
• Catalog: ab2426
• Prices: $400.00
• Sizes: 1 ml
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F IP WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Hamster, Dog, Human, Zebrafish, Marmoset, Fish
• Antigen: Synthetic peptide: DMGHLKYYLAPKIEDEEGS , corresponding to C terminal amino acids 243-261 of Human PCNA
• Description: Rabbit Polyclonal
• Gene: PCNA
• Conjugate: Unconjugated

Product images

ab2426 staining PCNA in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH 9. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

ab2426 staining PCNA in murine skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TRIS EDTA pH 8.2. Samples were then incubated with the primary antibody at a 1/2000 dilution for 1 hour at 37°C. An undiluted HRP-conjugated rabbit polyclonal was used as secondary antibody.See Abreview

ab2426 staining PCNA in rat tumor tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

ab2426 was used for immunohistochemistry, at a 1:500 dilution in hamster neural tissue, identified with a polyclonal secondary and DAB detection kit. Review by Kevin Bath submitted 18 June 2004

ab2426 at 1/200 dilution staining rat skeletal muscle satellite stem cells by ICC/IF.Cultured rat skeletal muscle satellite stem cells (SKMB) were 2% paraformaldehyde fixed for 15 minutes and then permealized with Triton-X100 prior to incubation with ab2426 overnight at 4°C. A donkey anti-rabbit Alexa-Fluor ® 488 (ab150073) antibody was used as the secondary.The image shows DAPI (blue-nuclear stain, upper left panel), PCNA (Green, upper right panel, showing nuclear localization in actively dividing cells), same SKMB cells -DIC (phase) image (lower left panel) and superimpose fluorescence image (lower right panel).See Abreview

ab2426 staining PCNA in murine epithelial tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Wound tissue sections (0.5 µm) were cut using a microtome and collected on slides. Sections were then de-waxed in xylene and rehydrated by successive immersion in descending concentrations of alcohol. The sections were then subjected for immunofluorescence staining. Briefly, tissue sections were blocked by incubated with 5% donkey serum (ab7475) for 1 hour and washed with phosphate-buffered saline (PBS). Sections were then incubated with ab2426 at a 1/500 for 1 hour at room temperature under humidified conditions. After primary antibody incubation, sections were washed with PBS and incubated with appropriate fluorescent secondary antibodies for 1 hour at room temperature. Sections were then washed with PBS, mounted with mounting medium containing DAPI.

All lanes : Anti-PCNA antibody (ab2426) at 1/200 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution

Anti-PCNA antibody (ab2426) at 1/1000 dilution + 293T whole cell lysate at 70 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-PCNA antibody (ab2426) at 1/300 dilutionLane 1 : Whole tissue lysate prepared from mouse heartLane 2 : Whole tissue lysate prepared from mouse heartLane 3 : Whole tissue lysate prepared from mouse heartLane 4 : Whole tissue lysate prepared from mouse heartLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated pig anti-rabbit polyclonal at 1/3000 dilutiondeveloped using the ECL technique

Product References

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Inserra PI, Leopardo NP, Willis MA, Freysselinard AL, Vitullo AD. Reproduction. 2013 Dec 20;147(2):199-209.

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Barone M, Notarnicola M, Caruso MG, Scavo MP, Viggiani MT, Tutino V, Polimeno L, Pesetti B, Di Leo A, Francavilla A. Carcinogenesis. 2014 Jul;35(7):1613-9.

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Gao J, Wang F, Wang W, Su Z, Guo C, Cao S. PLoS One. 2014 Apr 1;9(4):e93588.

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Brown DI, Lassegue B, Lee M, Zafari R, Long JS, Saavedra HI, Griendling KK. PLoS One. 2014 May 5;9(5):e96657.

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Altamemi I, Murphy EA, Catroppo JF, Zumbrun EE, Zhang J, McClellan JL, Singh UP, Nagarkatti PS, Nagarkatti M. J Pharmacol Exp Ther. 2014 Jul;350(1):99-109.

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Qing H, Liu Y, Zhao Y, Aono J, Jones KL, Heywood EB, Howatt D, Binkley CM, Daugherty A, Liang Y, Bruemmer D. Stem Cells. 2014 Sep;32(9):2419-29.

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Papapietro O, Teatero S, Thanabalasuriar A, Yuki KE, Diez E, Zhu L, Kang E, Dhillon S, Muise AM, Durocher Y, Marcinkiewicz MM, Malo D, Gruenheid S. Nat Commun. 2013;4:1898.

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Jia C, Hayoz S, Hutch CR, Iqbal TR, Pooley AE, Hegg CC. PLoS One. 2013;8(3):e58668.