PCNA

Gene Symbol PCNA
Entrez Gene 5111
Alt Symbol ATLD2
Species Human
Gene Type protein-coding
Description proliferating cell nuclear antigen
Other Description DNA polymerase delta auxiliary protein|cyclin
Swissprots D3DW02 B2R897 P12004
Accessions AAA60040 AAM78556 AAP58729 AAP58730 AAP58731 AAP58732 EAX10428 EAX10429 EAX10430 P12004 AK300558 BAG62264 AK311014 AK313286 BAG36094 BC000491 AAH00491 BC062439 AAH62439 BG612192 BU626265 CB990423 CR536501 CAG38740 CR541799 CAG46598 D28458 BAA05824 DQ891403 ABM82329 DQ894581 ABM85507 M15796 AAA35736 NM_002592 NP_002583 NM_182649 NP_872590
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. {ECO:0000269|PubMed:18719106
Subcellular Location Nucleus {ECO:0000269|PubMed:24115439}. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Top Pathways DNA replication, Mismatch repair, Cell cycle, Base excision repair, Nucleotide excision repair

PCNA (PC10) Mouse mAb (PE Conjugate) - 8607 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone PC10
Flow cytometric analysis of Jurkat cells pre-extracted with Triton-X-100 (see protocol) using PCNA (PC10) Mouse mAb (PE Conjugate) and DRAQ5® #4084 (DNA content).

PCNA (PC10) Mouse mAb (Alexa Fluor ® 488 Conjugate) - 8580 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone PC10
Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (Alexa Fluor ® 488 Conjugate) (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 647 Conjugate) #8587 (blue pseudocolor), and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) #3475 (red).

Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb - 13439 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C7P
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (100 mJ, 1 hr recovery; +), using Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (upper) or PCNA (PC10) Mouse mAb #2586 (lower).

PCNA (D3H8P) XP ® Rabbit mAb - 13110 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3H8P
Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP ® Rabbit mAb.

PCNA (PC10) Mouse mAb - 2586 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Mouse
Clonality Monoclonal
Clone PC10
Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.

PCNA (F-2) - sc-25280 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (F-2): sc-25280. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A) and immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tumor (B) showing nuclear localization.

PCNA (FL-261) - sc-7907 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.

PCNA (PC11) - sc-53407 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (PC11): sc-53407. Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.

PCNA (C-20) - sc-9857 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PCNA (C-20): sc-9857. Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.

PCNA (PC10) - sc-56 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (PC10): sc-56. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tumor showing strong nuclear staining of hepatocytes (B).

PCNA (PC5) - sc-53408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (PC5): sc-53408. Western blot analysis of PCNA expression in A-431 (A), MOLT-4 (B), HeLa (C) and CCRF-CEM (D) whole cell lysates.

PCNA (SPM350) - sc-65598 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (SPM350): sc-65598. Western blot analysis of PCNA expression in A-431 (A), NIH/3T3 (B), MOLT-4 (C) and HeLa (D) whole cell lysates and Hep G2 (E) and HeLa (F) nuclear extracts.

PCNA (6D645) - sc-71858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PCNA (6D645): sc-71858. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates.

Anti-PCNA (phospho Y211) antibody - ab85000 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab85000 at 1/250 dilution (incubated for 18 hours at room temperature) staining PCNA in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).

Anti-PCNA antibody - ab2426 from Abcam

Prices $400.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab2426 staining PCNA in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH 9. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-PCNA antibody - ab15497 from Abcam

Prices $400.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15497/ab> staining PCNA in Human tonsil tissue (ab29614) by Immunohistochemisty (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PCNA antibody - ab152112 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilutionLane 1 : HCT116 whole cell lysate at 15 µgLane 2 : HCT116 whole cell lysate at 10 µgLane 3 : HCT116 whole cell lysate at 5 µgLane 4 : HCT116 whole cell lysate at 1 µg

Anti-PCNA antibody - ab53048 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PCNA antibody (ab53048) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with immunising peptide

Anti-PCNA antibody - ab34747 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-PCNA antibody [216 (PC10)] - ab115964 from Abcam

Host Mouse
Clonality Monoclonal
Clone 216 (PC10)

Anti-PCNA antibody [2E1-G10-H10] - ab184660 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E1-G10-H10
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb [2E1-G10-H10] diluted 1:400,showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).

Anti-PCNA antibody [339] - ab12449 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 339

Anti-PCNA antibody [EPR3821] - ab92552 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3821
All lanes : Anti-PCNA antibody [EPR3821] (ab92552) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PCNA antibody [EPR3821] (Alexa Fluor® 488) - ab193963 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3821
ab193963 staining PCNA in HeLa cells. The cells were fixed with 100% methanol (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab193963 at a working dilution of 1/100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

Anti-PCNA antibody [EPR3821] (Alexa Fluor® 647) - ab193964 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3821
ab193964 staining PCNA in HeLa cells. The cells were fixed with 100% methanol (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab193964 at a working diltuion of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 488 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).
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