Anti-PCNA antibody

• Supplier: Abcam
• Catalog: ab15497
• Prices: $400.00
• Sizes: 500 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein corresponding to Human PCNA
• Description: Rabbit Polyclonal
• Gene: PCNA
• Conjugate: Unconjugated

Product images

ab15497/ab> staining PCNA in Human tonsil tissue (ab29614) by Immunohistochemisty (Formalin/PFA-fixed paraffin-embedded sections).

ICC/IF image of ab15497/ab> stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15497/ab>, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PCNA antibody (ab15497) at 1/1000 dilution + Mouse heart tissue lysate at 30 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-PCNA antibody (ab15497) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 conjugated goat anti-rabbit IgG (H&L) at 1/15000 dilution

Anti-PCNA antibody (ab15497) at 1/25 dilution + Raji cell lysate

Product References

Fibromodulin deficiency reduces low-density lipoprotein accumulation in - Fibromodulin deficiency reduces low-density lipoprotein accumulation in

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Muscle degeneration in neuraminidase 1-deficient mice results from infiltration - Muscle degeneration in neuraminidase 1-deficient mice results from infiltration

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Morphine induces mesangial cell proliferation and glomerulopathy via kappa-opioid - Morphine induces mesangial cell proliferation and glomerulopathy via kappa-opioid

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PCNA interacts with Prox1 and represses its transcriptional activity. - PCNA interacts with Prox1 and represses its transcriptional activity.

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