Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb [2E1-G10-H10] diluted 1:400,showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
Detection of Human PCNA by Western Blot of Immunoprecipitate of HeLa cell lysate.Lane 1: No IPLane 2: Control IgG IPLane 3: ab184660 IP
All lanes : Anti-PCNA antibody [2E1-G10-H10] (ab184660) at 1/1000 dilutionLane 1 : CHO-K1 lysateLane 2 : C6 lysateLane 3 : COS7 lysateLane 4 : 3T3 lysateLane 5 : HeLa lysate
Immunofluorescent anaysis of HeLa cells (100% methanol-fixed, 2 hours at -20ºC) labeling PCNA with ab184660 at 1/100 dilution.