Anti-PCNA antibody [EPR3821]

• Supplier: Abcam
• Catalog: ab92552
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3821
• Applications: WB IP ICC/IF IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human PCNA (UniProt ID: P12004)
• Description: Rabbit Monoclonal
• Gene: PCNA
• Conjugate: Unconjugated

Product images

All lanes : Anti-PCNA antibody [EPR3821] (ab92552) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Overlay histogram showing HeLa cells stained with ab92552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92552, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab92552 (1/200) staining PCNA in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.See Abreview

ab92552 at 1/100 dilution staining PCNA in Tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

ab92552 at 1/100 dilution staining PCNA in HeLa cells by Immunofluorescence.

ab92552 showing positive staining in Ovarian carcinoma tissue.

ab92552 showing negative staining in Normal brain tissue.

ab92552 showing positive staining in Urinary bladder carcinoma tissue.

ab92552 showing positive staining in Normal stomach tissue.

ab92552 showing positive staining in Normal colon tissue.

ab92552 showing positive staining in Breast carcinoma tissue.

ab92552 showing positive staining in Glioma tissue.

Product References

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