Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PCQAP / MED15 with ab181158 at 1/50 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling PCQAP / MED15 with ab181158 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PCQAP / MED15 with ab181158 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution