MED15

Gene Symbol	MED15
Entrez Gene	51586
Alt Symbol	ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7
Species	Human
Gene Type	protein-coding
Description	mediator complex subunit 15
Other Description	CTG repeat protein 7a\|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein\|PC2 glutamine/Q-rich-associated protein\|PC2-glutamine-rich-associated protein\|TPA inducible gene-1\|TPA inducible protein\|TPA-inducible gene 1 protein\|activator-recruited cofactor 105 kDa component\|activator-recruited cofactor, 105-kD\|mediator of RNA polymerase II transcription subunit 15\|positive cofactor 2 glutamine/Q-rich-associated protein\|positive cofactor 2, glutamine/Q-rich-associated protein\|trinucleotide repeat containing 7\|trinucleotide repeat-containing gene 7 protein
Swissprots	Q96IH7 O15413 Q96RN5 Q6IC31 D3DX31 Q9P1T3 D3DX32 Q8NF16 Q96CT0
Accessions	EAX02948 EAX02949 EAX02950 EAX02951 EAX02952 EAX02953 EAX02954 EAX02955 EAX02956 Q96RN5 AF056191 AAC12944 AF328769 AAK58423 AK000003 BAA92228 AK074230 AK074268 BAB85034 AK090465 BAC03446 AK124276 BAG54024 AK124925 BAG54113 AK126208 AK294540 BAG57747 AK294734 BAH11863 AK297284 BAG59755 AK297789 BAG60130 AK315540 AL832151 CAH10580 AL832923 CAH10623 AW245653 BC003078 AAH03078 BC005027 BC007529 AAH07529 BC013985 AAH13985 BC017110 BX478424 CB156495 CB988805 CR456537 CAG30423 CU013137 CAK54568 CU013425 CAK54867 DA182752 KF274544 KF274593 U80745 AAB91443 XM_006724263 XP_006724326 XM_006724264 XP_006724327 XM_011530214 XP_011528516 XM_011530215 XP_011528517 XM_011530216 XP_011528518 XM_011530217 XP_011528519 XM_011530218 XP_011528520 XM_011530219 XP_011528521 XM_011530220 XP_011528522 XM_011530221 XP_011528523 NM_001003891 NP_001003891 NM_001293234 NP_001280163 NM_001293235 NP_001280164 NM_001293236 NP_001280165 NM_001293237 NP_001280166 NM_015889 NP_056973
Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. {ECO:0000269\|PubMed:12167862, ECO:0000269\|PubMed:16630888, ECO:0000269\|PubMed:16799563}.
Subcellular Location	Cytoplasm. Nucleus.
Tissue Specificity	Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney. {ECO:0000269\|PubMed:11024300}.

Med15 (D-20) - sc-86728 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Med15 (H-178) - sc-292267 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Med15 (KJ-2) - sc-101185 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

Med15 (KJ-2): sc-101185. Western blot analysis of Med15 expression in HeLa nuclear extract.

Med15 (L-17) - sc-86730 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-PCQAP / MED15 antibody - ab176593 from Abcam

Prices	$428.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PCQAP / MED15 with ab176593 at 1/250 dilution. Detection: DAB staining.

Anti-PCQAP / MED15 antibody - ab192496 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PCQAP / MED15 antibody (ab192496)Lane 1 : MCF7 whole cell lysateLane 2 : NIH 3T3 whole cell lysateLane 3 : PC12 whole cell lysate

Anti-PCQAP / MED15 antibody - ab81969 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-PCQAP / MED15 antibody (ab81969) at 1 µg/ml + human lung lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-PCQAP / MED15 antibody - ab57171 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-PCQAP / MED15 antibody [EPR13309] - ab181158 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13309

Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PCQAP / MED15 with ab181158 at 1/50 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Anti-MED15 / ARC105 Antibody (aa650-700) - LS-C289161 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED15 / ARC105 Antibody (aa1-89, Azide-free) - LS-C327863 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal

Anti-MED15 / ARC105 Antibody (aa319-332) - LS-C87401 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody (1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-MED15 / ARC105 Antibody - LS-C340991 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MED15 / ARC105 Antibody (aa393-679, clone 1A2) - LS-C340412 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1A2

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.

Anti-MED15 / ARC105 Antibody (aa393-679, clone 2H6) - LS-C340410 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2H6

Anti-MED15 / ARC105 Antibody (aa393-679, clone 1D5) - LS-C340413 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1D5

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MED15 / ARC105 Antibody (C-Terminus) - LS-C345847 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of MED15 Antibody with human Fetal Liver lysate.

Anti-MED15 / ARC105 Antibody (aa450-500) - LS-C286118 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human MED15 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MED15 used at a dilution of 1:200 (1 Detection: DAB.

Anti-MED15 / ARC105 Antibody (aa450-500) - LS-C286883 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED15 / ARC105 Antibody (aa393-679, clone 1H5) - LS-C340416 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1H5

IHC of paraffin-embedded Human endometrium tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

MED15 Antibody - NBP1-89018 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: MED15 Antibody [NBP1-89018] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).

MED15 Antibody (4A4) - H00051586-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4A4

Western Blot: MED15 Antibody (4A4) [H00051586-M02] - Western Blot analysis of PCQAP expression in NIH/3T3(Cat # L018V1 ).

MED15 Antibody - NBP1-28800 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: MED15 Antibody [NBP1-28800] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MED15 Antibody - NBP2-37851 from Novus Biologicals

Prices	$139.00, $369.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: MED15 Antibody [NBP2-37851] - Fetal Liver lysates, Antibody Dilution: 1.0 ug/ml.

Anti-PCQAP/MED15 antibody produced in goat - SAB2500761 from Sigma-Aldrich

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-PCQAP / MED15 : <B>Cat. No. SAB2500761</B>: (1 μg/mL) staining of Human Lung lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Search more