Anti-PCSK9 antibody
| Name | Anti-PCSK9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28770 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: CRSRHLAQASQELQ , corresponding to C terminal amino acids 679-692 of Human PCSK9 Run BLAST with Run BLAST with |
| Description | Goat Polyclonal |
| Gene | PCSK9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PCSK9 antibody (ab28770) at 0.2 µg/mlLane 1 : untransfected McA cell lysate.Lane 2 : McA cell lysate transfected with wild type human PCSK9 Lane 3 : McA cell lysate transfected with S127R human PCSK9
ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, staining PCSK9 with ab28770 at 4 µg/ml. Staining was detected using HRP. Antigen retrieval was by heat mediation in TRIS/EDTA buffer (pH 9).
Anti-PCSK9 antibody (ab28770) at 0.3 µg/ml + Human Adipose lysate in RIPA buffer at 35 µgdeveloped using the ECL technique
Product References
PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult - PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult
Liu M, Wu G, Baysarowich J, Kavana M, Addona GH, Bierilo KK, Mudgett JS, Pavlovic G, Sitlani A, Renger JJ, Hubbard BK, Fisher TS, Zerbinatti CV. J Lipid Res. 2010 Sep;51(9):2611-8.
Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic - Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic
Lambert G, Ancellin N, Charlton F, Comas D, Pilot J, Keech A, Patel S, Sullivan DR, Cohn JS, Rye KA, Barter PJ. Clin Chem. 2008 Jun;54(6):1038-45.
A new PCSK9 gene promoter variant affects gene expression and causes autosomal - A new PCSK9 gene promoter variant affects gene expression and causes autosomal
Blesa S, Vernia S, Garcia-Garcia AB, Martinez-Hervas S, Ivorra C, Gonzalez-Albert V, Ascaso JF, Martin-Escudero JC, Real JT, Carmena R, Casado M, Chaves FJ. J Clin Endocrinol Metab. 2008 Sep;93(9):3577-83.