All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/10000 dilutionLane 1 : A431 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/2000 dilution + Human fetal liver lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using ab181142 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.