Anti-PCSK9 antibody [mAbcam84041]

• Supplier: Abcam
• Catalog: ab84041
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone: mAbcam84041
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human, Primate
• Antigen: Recombinant fragment derived from within residues 100 - 200 of Human PCSK9
• Description: Mouse Monoclonal
• Gene: PCSK9
• Conjugate: Unconjugated

Product images

All lanes : Anti-PCSK9 antibody [mAbcam84041] (ab84041) at 1/500 dilutionLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgM (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab84041 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84041, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.