Flow cytometric analysis of BxPC-3 cells (2% paraformaldehyde-fixed) labeling PCTAIRE1 with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
All lanes : Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilutionLane 1 : Human testis cell lysateLane 2 : Human fetal heart cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution + BxPC-3 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution