Anti-PD-L1 antibody
| Name | Anti-PD-L1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58810 |
| Prices | $382.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide corresponding to Human PD-L1 |
| Description | Rabbit Polyclonal |
| Gene | CD274 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-PD-L1 antibody (ab58810) at 0.5 µg/mlLane 2 : Anti-PD-L1 antibody (ab58810) at 1 µg/mlLane 1 : Raji cell lysateLane 2 : Raji cell lysateLysates/proteins at 15 µg per lane.
IHC image of ab58810 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
High level of soluble programmed cell death ligand 1 in blood impacts overall - High level of soluble programmed cell death ligand 1 in blood impacts overall
Rossille D, Gressier M, Damotte D, Maucort-Boulch D, Pangault C, Semana G, Le Gouill S, Haioun C, Tarte K, Lamy T, Milpied N, Fest T. Leukemia. 2014 Dec;28(12):2367-75.
PD-L1 is a novel direct target of HIF-1alpha, and its blockade under hypoxia - PD-L1 is a novel direct target of HIF-1alpha, and its blockade under hypoxia
Noman MZ, Desantis G, Janji B, Hasmim M, Karray S, Dessen P, Bronte V, Chouaib S. J Exp Med. 2014 May 5;211(5):781-90.
Author's response to "Letter to the editor: unvalidated antibodies and misleading - Author's response to "Letter to the editor: unvalidated antibodies and misleading
Muenst S, Tzankov A, Gillanders WE, Soysal SD. Breast Cancer Res Treat. 2014 Sep;147(2):459-62.
Gliomas promote immunosuppression through induction of B7-H1 expression in - Gliomas promote immunosuppression through induction of B7-H1 expression in
Bloch O, Crane CA, Kaur R, Safaee M, Rutkowski MJ, Parsa AT. Clin Cancer Res. 2013 Jun 15;19(12):3165-75.
B7-H1 expression is associated with poor prognosis in colorectal carcinoma and - B7-H1 expression is associated with poor prognosis in colorectal carcinoma and
Shi SJ, Wang LJ, Wang GD, Guo ZY, Wei M, Meng YL, Yang AG, Wen WH. PLoS One. 2013 Oct 4;8(10):e76012.
Local blockade of epithelial PDL-1 in the airways enhances T cell function and - Local blockade of epithelial PDL-1 in the airways enhances T cell function and
McNally B, Ye F, Willette M, Flano E. J Virol. 2013 Dec;87(23):12916-24.
Immunological and molecular correlates of disease recurrence after liver - Immunological and molecular correlates of disease recurrence after liver
Cariani E, Pilli M, Zerbini A, Rota C, Olivani A, Pelosi G, Schianchi C, Soliani P, Campanini N, Silini EM, Trenti T, Ferrari C, Missale G. PLoS One. 2012;7(3):e32493.