CD274

Gene Symbol CD274
Entrez Gene 29126
Alt Symbol B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Species Human
Gene Type protein-coding
Description CD274 molecule
Other Description B7 homolog 1|CD274 antigen|PDCD1 ligand 1|programmed cell death 1 ligand 1|programmed death ligand 1
Swissprots Q9NUZ5 Q6WEX4 B4DU27 Q14CJ2 Q2V8D5 Q9NZQ7 Q66RK1 B2RBA2
Accessions CCF77002 EAW58763 Q9NZQ7 AF177937 AAF25807 AF233516 AAG18508 AI826502 AK001894 BAA91966 AK300470 BAG62189 AK314567 BAG37149 AY254342 AAP13470 AY291313 AAP42144 AY714881 AAU09634 BC069381 AAH69381 BC074984 AAH74984 BC113734 AAI13735 BC113736 AAI13737 DQ286582 ABB90152 DQ836393 ABI16084 HQ258151 ADR82905 XM_006716759 XP_006716822 NM_001267706 NP_001254635 NM_014143 NP_054862 NR_052005
Function Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. {ECO:0000269|PubMed:10581077, ECO:0000269|PubMed:11015443}.
Subcellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. {ECO:0000269|PubMed:10581077, ECO:0000269|PubMed:11015443}.
Top Pathways Cell adhesion molecules (CAMs)

PD-L1 (E1L3N ® ) XP ® Rabbit mAb - 13684 from Cell Signaling Technology

Prices $129.00, $397.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Western blot analysis of extracts from KARPAS-299, SUP-M2, and PC-3 cells using PD-L1 (E1L3N ® ) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Pdcd-1L1 (H-130) - sc-50298 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pdcd-1L1 (H-130): sc-50298. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.

Pdcd-1L1 (N-20) - sc-19090 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pdcd-1L1 (T-20) - sc-19091 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pdcd-1L1 (T-20): sc-19091. Western blot analysis of Pdcd-1L1 expression in K-562 (A) and AML-193 (B) whole cell lysates.

Anti-PD-L1 antibody - ab58810 from Abcam

Prices $382.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PD-L1 antibody (ab58810) at 0.5 µg/mlLane 2 : Anti-PD-L1 antibody (ab58810) at 1 µg/mlLane 1 : Raji cell lysateLane 2 : Raji cell lysateLysates/proteins at 15 µg per lane.

Anti-PD-L1 antibody - ab153991 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PD-L1 antibody (ab153991) at 1/500 dilutionLane 1 : A549Lane 2 : HeLaLane 3 : HepG2Lane 4 : HCT116Lysates/proteins at 30 µg per lane.

Anti-PD-L1 antibody - ab156361 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
IHC image of PD-L1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156361, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PD-L1 antibody - ab28753 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PD-L1 antibody (ab28753) at 1 µg/ml + Human Heart lysate at 35 µg

Anti-PD-L1 antibody [1B12] - ab180370 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B12
All lanes : Anti-PD-L1 antibody [1B12] (ab180370) at 1/4000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD274 cDNALysates/proteins at 5 µg per lane.

Anti-PD-L1 antibody [EPR1161(2)] - ab174838 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1161(2)
All lanes : Anti-PD-L1 antibody [EPR1161(2)] (ab174838) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : K562 cell lysateLane 3 : Raji cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-PD-L1 antibody [MIH2] - ab109052 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MIH2
Staining of human peripheral blood monocytes with Mouse anti Human CD274.

Anti-PD-L1 antibody [MIH2] (Phycoerythrin) - ab80391 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MIH2
ab80391 at 10µl/106 cells staining CD274 in human peripheral blood monocytes by Flow cytometry.

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone 29E.2A3) - LS-C40827 from LifeSpan Bioscience

Prices $225.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 29E.2A3
Human peripheral blood lymphocytes stained with purified 29E.2A3, followed by biotinylated anti-mouse IgG and Sav-PE

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH6) - LS-C58199 from LifeSpan Bioscience

Prices $235.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone MIH6
Mouse lymphocytes stained with Rat anti Mouse CD274.

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH6, FITC) - LS-C58198 from LifeSpan Bioscience

Prices $340.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone MIH6
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC.

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH2) - LS-C58200 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MIH2
Peripheral Human lymphocytes stained with Mouse anti Human CD274.

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH6) - LS-C123195 from LifeSpan Bioscience

Prices $435.00
Sizes 250 µg
Host Rat
Clonality Monoclonal
Clone MIH6

Anti-CD274 / B7-H1 / PD-L1 Antibody (PE) - LS-C125334 from LifeSpan Bioscience

Prices $475.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-CD274 / B7-H1 / PD-L1 Antibody (FITC) - LS-C128033 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CD274 / B7-H1 / PD-L1 Antibody (aa19-338, clone 130021) - LS-C128031 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 130021

Anti-CD274 / B7-H1 / PD-L1 Antibody (aa166-176) - LS-C54629 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH3) - LS-C179364 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MIH3

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone 27A2, PE) - LS-C179482 from LifeSpan Bioscience

Prices $350.00
Sizes 50 tst
Host Mouse
Clonality Monoclonal
Clone 27A2

Anti-CD274 / B7-H1 / PD-L1 Antibody (aa19-238, clone 12K56, APC) - LS-C182804 from LifeSpan Bioscience

Prices $635.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal
Clone 12K56

Anti-CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1, Azide-free) - LS-C134638 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ANC6H1
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
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