All lanes : Anti-PDE2A antibody (ab125677) at 1/2000 dilutionLane 1 : COS cells, non- transfectedLane 2 : COS cells, transfected with Human PDE1A3Lane 3 : COS cells transfected with Human PDE2A3Lane 4 : COS cells, transfected with Human PDE3ALane 5 : COS cells, transfected with Human PDE3BLane 6 : COS cells, transfected with Human PDE4D2Lane 7 : COS cells, transfected with Human PDE5A1Lane 8 : COS cells, transfected with Human PDE5A3Lane 9 : COS cells, transfected with Human PDE7A1Lane 10 : COS cells transfected with Human PDE8A1Lane 11 : COS cells, transfected with Human PDE10A1Lane 12 : COS cells, transfected with Human PDE11A1developed using the ECL technique