Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody recognising an upstream epitope of PDE3B. Lane 2: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99290 at 3 µg/mg lysate for IP. Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG. Subsequent Western blot detection used ab99290 at 1 µg/ml. Developed using the ECL technique with an exposure time of 10 seconds.