Anti-PDE4 antibody
| Name | Anti-PDE4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14628 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) C terminal |
| Description | Rabbit Polyclonal |
| Gene | PDE4A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Increased PKA signaling disrupts recognition memory and spatial memory: role in - Increased PKA signaling disrupts recognition memory and spatial memory: role in
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Equilibrium between adenylyl cyclase and phosphodiesterase patterns adrenergic - Equilibrium between adenylyl cyclase and phosphodiesterase patterns adrenergic
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Maternal exposure to secondhand cigarette smoke primes the lung for induction of - Maternal exposure to secondhand cigarette smoke primes the lung for induction of
Singh SP, Mishra NC, Rir-Sima-Ah J, Campen M, Kurup V, Razani-Boroujerdi S, Sopori ML. J Immunol. 2009 Aug 1;183(3):2115-21.
Differential association of phosphodiesterase 4D isoforms with beta2-adrenoceptor - Differential association of phosphodiesterase 4D isoforms with beta2-adrenoceptor
De Arcangelis V, Liu R, Soto D, Xiang Y. J Biol Chem. 2009 Dec 4;284(49):33824-32.
Phosphodiesterase 4 and phosphatase 2A differentially regulate cAMP/protein - Phosphodiesterase 4 and phosphatase 2A differentially regulate cAMP/protein
De Arcangelis V, Soto D, Xiang Y. Mol Pharmacol. 2008 Nov;74(5):1453-62.
Chronic ethanol feeding suppresses beta-adrenergic receptor-stimulated lipolysis - Chronic ethanol feeding suppresses beta-adrenergic receptor-stimulated lipolysis
Kang L, Nagy LE. Endocrinology. 2006 Sep;147(9):4330-8. Epub 2006 Jun 22.