Anti-PDE4D antibody

• Supplier: Abcam
• Catalog: ab14613
• Prices: $390.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ICC/IF ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Hamster, Human
• Antigen: C-terminal cyclic peptide unique to all PDE4D subtypes variants
• Blocking Peptide: PDE4D peptide
• Description: Rabbit Polyclonal
• Gene: PDE4D
• Conjugate: Unconjugated

Product images

ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab14613 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14613, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot using ab14613 and positive controls for PDE4D variants.

Product References

Ischemia-induced increase in microvascular phosphodiesterase 4D expression in rat - Ischemia-induced increase in microvascular phosphodiesterase 4D expression in rat

He Z, He B, Behrle BL, Fejleh MP, Cui L, Paule MG, Greenfield LJ. ACS Chem Neurosci. 2012 Jun 20;3(6):428-32.

Cyclic nucleotide signaling in polycystic kidney disease. - Cyclic nucleotide signaling in polycystic kidney disease.

Wang X, Ward CJ, Harris PC, Torres VE. Kidney Int. 2010 Jan;77(2):129-40.

Compartmentalized cyclic adenosine 3',5'-monophosphate at the plasma membrane - Compartmentalized cyclic adenosine 3',5'-monophosphate at the plasma membrane

Penmatsa H, Zhang W, Yarlagadda S, Li C, Conoley VG, Yue J, Bahouth SW, Buddington RK, Zhang G, Nelson DJ, Sonecha MD, Manganiello V, Wine JJ, Naren AP. Mol Biol Cell. 2010 Mar 15;21(6):1097-110.

Identification of PDE4D as a proliferation promoting factor in prostate cancer - Identification of PDE4D as a proliferation promoting factor in prostate cancer

Rahrmann EP, Collier LS, Knutson TP, Doyal ME, Kuslak SL, Green LE, Malinowski RL, Roethe L, Akagi K, Waknitz M, Huang W, Largaespada DA, Marker PC. Cancer Res. 2009 May 15;69(10):4388-97.

Expression and activity of phosphodiesterase isoforms during epithelial - Expression and activity of phosphodiesterase isoforms during epithelial

Kolosionek E, Savai R, Ghofrani HA, Weissmann N, Guenther A, Grimminger F, Seeger W, Banat GA, Schermuly RT, Pullamsetti SS. Mol Biol Cell. 2009 Nov;20(22):4751-65.

Dynamic regulation of cystic fibrosis transmembrane conductance regulator by - Dynamic regulation of cystic fibrosis transmembrane conductance regulator by

Lee JH, Richter W, Namkung W, Kim KH, Kim E, Conti M, Lee MG. J Biol Chem. 2007 Apr 6;282(14):10414-22. Epub 2007 Jan 23.

An anchored PKA and PDE4 complex regulates subplasmalemmal cAMP dynamics. - An anchored PKA and PDE4 complex regulates subplasmalemmal cAMP dynamics.

Willoughby D, Wong W, Schaack J, Scott JD, Cooper DM. EMBO J. 2006 May 17;25(10):2051-61. Epub 2006 Apr 27.