PDE4D

Gene Symbol PDE4D
Entrez Gene 5144
Alt Symbol ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1
Species Human
Gene Type protein-coding
Description phosphodiesterase 4D, cAMP-specific
Other Description cAMP-specific 3',5'-cyclic phosphodiesterase 4D|cAMP-specific phosphodiesterase PDE4D6|phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
Swissprots Q8IVD3 Q8IVD2 Q9HCX7 Q13551 Q96HL4 Q8IVA9 Q13550 Q08499 Q8IV84 Q7Z2L8 O43433 Q13549
Accessions EAW54982 EAW54983 EAW54984 EAW54985 EAW54986 EAW54987 EAW54988 EAW54989 EAW54990 EAW54991 EAW54992 Q08499 AF012073 AAC00069 AF012074 AAC00070 AF536975 AAN10117 AF536976 AAN10118 AF536977 AAN10119 AF536980 AJ250852 CAC03756 AJ250854 CAC03757 AJ250855 CAC03758 AK307363 AY245866 AAP75760 AY245867 AAP75761 AY388960 AAQ90404 BC008390 AAH08390 BC036319 AAH36319 BF197530 BI492585 BT007398 AAP36062 BX648604 DB230503 JF432192 ADZ15409 L20970 AAA03592 U02882 AAC13745 U50157 AAA97890 U50158 AAA97891 U50159 AAA97892 U79571 AAC51928 XM_005248537 XP_005248594 XM_005248538 XP_005248595 XM_011543469 XP_011541771 XM_011543470 XP_011541772 XM_011543471 XP_011541773 XM_011543472 XP_011541774 XM_011543473 XP_011541775 XM_011543474 XP_011541776 XM_011543475 XP_011541777 XM_011543476 XP_011541778 XM_011543477 XP_011541779 XM_011543478 XP_011541780 XM_011543479 XP_011541781 NM_001104631 NP_001098101 NM_001165899 NP_001159371 NM_001197218 NP_001184147 NM_001197219 NP_001184148 NM_001197220 NP_001184149 NM_001197221 NP_001184150 NM_001197222 NP_001184151 NM_001197223 NP_001184152 NM_006203 NP_006194
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. {ECO:0000269|PubMed:15260978, ECO:0000269|PubMed:15576036}.
Subcellular Location Apical cell membrane {ECO:0000269|PubMed:14500724}. Cytoplasm {ECO:0000250}. Membrane {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells. {ECO:0000250}.
Tissue Specificity Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. {ECO:0000269|PubMed:12834813, ECO:0000269|PubMed:17244609}.
Top Pathways Morphine addiction, cAMP signaling pathway, Purine metabolism

PDE4D (H-69) - sc-25814 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PDE4D (H-69): sc-25814. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

PDE4D (G-16) - sc-25097 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDE4D (G-16): sc-25097. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic and membrane staining of myocytes.

PDE4D (K-16) - sc-25100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDE4D (K-16): sc-25100. Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoskeletal localization.

PDE4D (P-16) - sc-25098 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDE4D (P-16): sc-25098. Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.

Anti-PDE4D (phospho S190) antibody - ab59212 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilutionLane 1 : K562 cell extract treated with H2O2 (100uM, 30mins)Lane 2 : K562 cell extract treated with H2O2 (100uM, 30mins) with immunising peptide

Anti-PDE4D antibody - ab14613 from Abcam

Prices $390.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-PDE4D antibody - ab77080 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-PDE4D antibody (ab77080) at 1 µg/mlLane 1 : COS cell lysates transfected with Human PDE4D isoform 1 Lane 2 : COS cell lysates transfected with Human PDE4D isoform 3Lane 3 : COS cell lysates, non-transfected Lysates/proteins at 25 µg per lane.

Anti-PDE4D antibody - ab112018 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of PDE4D expression in formalin-fixed, paraffin-embedded Human heart tissue using ab112018 at 5 µg/ml.

Anti-PDE4D antibody - ab59249 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDE4D antibody (ab59249) at 1/500 dilutionLane 1 : K562 cell extracts treated with H2O2 (100uM, 30mins)Lane 2 : K562 cell extracts treated with H2O2 (100uM, 30mins) with immunizing peptide

Anti-PDE4D antibody - ab87329 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDE4D antibody (ab87329) at 1 µg/mlLane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 2 : Lung (Human) Tissue Lysate Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PDE4D antibody - ab97796 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PDE4D antibody (ab97796) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

Anti-PDE4D antibody - ab99409 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDE4D antibody (ab99409) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-PDE4D antibody - ab99410 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDE4D antibody (ab99410) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-PDE4D antibody [EPR11823-29] - ab171749 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11823-29
All lanes : Anti-cardiac Troponin I antibody (ab1000) at 1/171749 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal brain lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.

Anti-PDE4D antibody [EPR11823-31] - ab171750 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11823-31
All lanes : Anti-PDE4D antibody [EPR11823-31] (ab171750) at 1/1000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal brain lysateLysates/proteins at 10 µg per lane.

Anti-PDE4D3 antibody - ab14614 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PDE4D3 antibody (ab14614) at 1/500 dilution + Active human PDE4D3 full length protein (ab80332) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 8 minutes

Anti-PDE4D3 antibody - ab59021 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-PDE4D3 antibody (ab59021) at 0.01 µg/ml + Rat Brain lysate (35µg protein in RIPA buffer)Observed band size : 77 kDa (why is the actual band size different from the predicted?)

Anti-PDE4D5 antibody - ab14626 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PDE4D7 antibody - ab14629 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PDE4D7 antibody (ab14629)Lanes 2 - 3 : Anti-PDE4D7 antibody (ab14629) at 1/750 dilutionLane 1 : Negative ControlLane 2 : Human Rec-PDE4D7 (1 ng)Lane 3 : Human Rec-PDE4D7 (10 ng)

Anti-PDE4D Antibody - LS-C331614 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human stomach cancer using PDE4D antibody at dilution of 1:200 (x400 lens).

Anti-PDE4D Antibody (aa746-809) - LS-C185640 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded H441 xenograft using PDE4D antibody at 1:100 dilution.

Anti-PDE4D Antibody (aa164-213) IHC-plus™ - LS-B8230 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PDE4D antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8230 dilution 2.5-5 ug/ml.

Anti-PDE4D Antibody (aa796-809) IHC-plus™ - LS-B3940 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PDE4D antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3940 concentration 5 ug/ml.

Anti-PDE4D Antibody (N-Terminus) - LS-C90172 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PDE4D Antibody (N-Terminus) - LS-C90174 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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