Anti-PDE5A antibody
| Name | Anti-PDE5A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14672 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic cyclic peptide (common to all PDE5A variants) (Rat) |
| Description | Rabbit Polyclonal |
| Gene | PDE5A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab14672 (4µg/ml) staining PDE5A in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in stratified squamous epithelium cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab14672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14672, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Low myocardial protein kinase G activity in heart failure with preserved ejection - Low myocardial protein kinase G activity in heart failure with preserved ejection
van Heerebeek L, Hamdani N, Falcao-Pires I, Leite-Moreira AF, Begieneman MP, Bronzwaer JG, van der Velden J, Stienen GJ, Laarman GJ, Somsen A, Verheugt FW, Niessen HW, Paulus WJ. Circulation. 2012 Aug 14;126(7):830-9.
Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant - Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant
Sogawa C, Abe A, Tsuji T, Koizumi M, Saga T, Kunieda T. Am J Pathol. 2010 Aug;177(2):822-8.
Expression of the PDE5 enzyme on human retinal tissue: new aspects of PDE5 - Expression of the PDE5 enzyme on human retinal tissue: new aspects of PDE5
Foresta C, Caretta N, Zuccarello D, Poletti A, Biagioli A, Caretti L, Galan A. Eye (Lond). 2008 Jan;22(1):144-9. Epub 2007 Jun 22.