All lanes : Anti-PDE9A antibody (ab125672) at 1/2000 dilutionLane 1 : Insect cells, transfected with Human PDE1A3Lane 2 : Insect cells transfected with Human PDE2A3Lane 3 : Insect cells, transfected with Human PDE3ALane 4 : Insect cells, transfected with Human PDE4A1Lane 5 : Insect cells, transfected with Human PDE5A2Lane 6 : Insect cells, transfected with Human PDE7ALane 7 : Insect cells, transfected with Human PDE8A1Lane 8 : Insect cells, transfected with Human PDE8BLane 9 : Insect cells transfected with Human PDE9A2Lane 10 : Insect cells, transfected with Human PDE10A1Lane 11 : Insect cells, transfected with Human PDE11A1developed using the ECL technique