All lanes : Anti-PDGF BB antibody (ab139753) at 1 µg/mlLane 1 : Human PDGF BB full length protein (ab73231)Lane 2 : Active PDGF BB full length protein (ab9706)Lysates/proteins at 0.01 µg per lane.SecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 8 minutes
IHC image of PDGF BB staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139753, 1µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.