Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGF Receptor alpha (phospho Y720) with ab134068. Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.
All lanes : Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/1000 dilutionLane 1 : NIH 3T3 cell lysate, untreatedLane 2 : NIH 3T3 cell lysate, treated with PervanadateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution