Anti-PDGF Receptor alpha (phospho Y742) antibody

• Supplier: Abcam
• Catalog: ab5452
• Prices: $378.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB
• Species Reactivities: Mouse, Rat, Human, Xenopus
• Antigen: Synthetic phospho peptide that contains Tyrosine 742 (Human)
• Description: Rabbit Polyclonal
• Gene: PDGFRA
• Conjugate: Unconjugated

Product images

Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5452 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5452 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Product References

Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. - Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas.

Paugh BS, Zhu X, Qu C, Endersby R, Diaz AK, Zhang J, Bax DA, Carvalho D, Reis RM, Onar-Thomas A, Broniscer A, Wetmore C, Zhang J, Jones C, Ellison DW, Baker SJ. Cancer Res. 2013 Oct 15;73(20):6219-29.

Efficient serum-free derivation of oligodendrocyte precursors from neural stem - Efficient serum-free derivation of oligodendrocyte precursors from neural stem

Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD. Stem Cells. 2009 Jan;27(1):116-25.