Anti-PDGF Receptor alpha (phospho Y754) antibody

• Supplier: Abcam
• Catalog: ab5460
• Prices: $394.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human, Rat, Xenopus
• Antigen: Synthetic phospho peptide (Human) containing Tyrosine 754
• Description: Rabbit Polyclonal
• Gene: PDGFRA
• Conjugate: Unconjugated

Product images

Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5460 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5460 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

ab5460 staining PDGF Receptor alpha (phospho Y754) in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H2O2, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes. See Abreview

ab5460 staining PDGF Receptor alpha (phospho Y754) in human glioblastoma multiforme brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H2O2, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes.See Abreview

Product References

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Johnson TV, DeKorver NW, Levasseur VA, Osborne A, Tassoni A, Lorber B, Heller JP, Villasmil R, Bull ND, Martin KR, Tomarev SI. Brain. 2014 Feb;137(Pt 2):503-19.

Investigation of the in vitro therapeutic efficacy of nilotinib in immortalized - Investigation of the in vitro therapeutic efficacy of nilotinib in immortalized

Sabha N, Au K, Agnihotri S, Singh S, Mangat R, Guha A, Zadeh G. PLoS One. 2012;7(6):e39412.