Anti-PDIA6 antibody
| Name | Anti-PDIA6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11432 |
| Prices | $390.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP ELISA WB |
| Species Reactivities | Mouse, Human, Pig, Rat, Hamster, Xenopus, Zebrafish |
| Antigen | Synthetic peptide corresponding to Human PDIA6 aa 476-492 |
| Description | Rabbit Polyclonal |
| Gene | PDIA6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![PDIA6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PDIA6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11432.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 48kDa; PDIA6](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/9291_ab11432-171830-IPV018ab114322min.jpg)
PDIA6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PDIA6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11432.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 48kDa; PDIA6
Western blot of PDIA6 in HT 1080 cell lysate using ab11432.
ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
.
An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic - An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic
Wang X, Lundgren AD, Singh P, Goodlett DR, Plymate SR, Wu JD. Biochem Biophys Res Commun. 2009 Sep 25;387(3):476-81. doi:
Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel - Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel
Viklund IM, Kuznetsov NV, Lofberg R, Daperno M, Sostegni R, Astegiano M, Rizzetto M, von Stein O, D'Amato M, von Stein P, Pettersson S. Int J Colorectal Dis. 2008 Oct;23(10):921-30.