Flow cytometric analysis of A431 cells labeling PDK2 with ab201964 at 1/200 dilution (green). Negative control (red).
All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : C6 cell lysateLane 3 : COS7 cell lysateLane 4 : K562 cell lysateLane 5 : A431 cell lysate
All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate
Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution + Human recombinant PDK2 (aa178-404) protein