Anti-PDK2 antibody [EP1947Y]

• Supplier: Abcam
• Catalog: ab76152
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1947Y
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues near the N terminus of human PDK2
• Description: Rabbit Monoclonal
• Gene: PDK2
• Conjugate: Unconjugated

Product images

ab76152 at 1/50 dilution staining PDK2 in human heart muscle by Immunohistochemistry, Paraffin-embedded tissue.

Anti-PDK2 antibody [EP1947Y] (ab76152) at 1/1000 dilution + SH-SY-5Y cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/1000 dilutiondeveloped using the ECL technique

Overlay histogram showing HepG2 cells stained with ab76152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76152, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.