Flow cytometric analysis of Raw26.7 cells (paraformaldehyde-fixed, 2%) labeling PDK3 with ab182574 at 1/400 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.
All lanes : Anti-PDK3 antibody [EP12309] (ab182574) at 1/2000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLane 3 : Rat heart lysateLane 4 : C6 lysateLane 5 : Raw264.7 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution