Anti-PDK4 antibody

• Supplier: Abcam
• Catalog: ab38242
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF ELISA WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to Human PDK4 aa 382-410 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH)
• Description: Rabbit Polyclonal
• Gene: PDK4
• Conjugate: Unconjugated

Product images

Fluorescent confocal image of HeLa cells stained with PDK4 (ab38242) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab38242 PDK4 () primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.

Immunohistochemical staining of PDK4 in Human adrenal tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab38242 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Immunohistochemical staining of PDK4 in Human skeletal muscle sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab38242. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-PDK4 antibody (ab38242) at 1/1000 dilution + mouse skeletal muscle tissue lysateSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution

ICC/IF image of ab38242 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38242, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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