PDK4

Gene Symbol PDK4
Entrez Gene 5166
Alt Symbol -
Species Human
Gene Type protein-coding
Description pyruvate dehydrogenase kinase, isozyme 4
Other Description [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial|pyruvate dehydrogenase kinase, isoenzyme 4|pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial
Swissprots Q16654
Accessions AAB67048 AAC50670 EAL24128 EAW76757 EAW76758 EAW76759 Q16654 AF334710 AK096428 BAG53287 AK098151 BAG53583 AK223328 BAD97048 AK312424 BAG35334 BC040239 AAH40239 DA923767 DB035430 DQ891256 ABM82182 DQ894441 ABM85367 U54617 AAC50669 NM_002612 NP_002603
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid
Subcellular Location Mitochondrion matrix.
Tissue Specificity Ubiquitous; highest levels of expression in heart and skeletal muscle. {ECO:0000269|PubMed:14966024, ECO:0000269|PubMed:21816445}.

PDK4 (C-16) - sc-14495 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PDK4 (N-15) - sc-14492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDK4 (N-15): sc-14492. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.

PDK4 (E-24) - sc-133899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
PDK4 (E-24): sc-133899. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.

PDK4 (P-24) - sc-130841 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PDK4 (P-24): sc-130841. Western blot analysis of PDK4 expression in non-transfected (A) and human PDK4 transfected (B) 293 whole cell lysates.

Anti-PDK4 antibody - ab38242 from Abcam

Host Rabbit
Clonality Polyclonal
Fluorescent confocal image of HeLa cells stained with PDK4 (ab38242) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab38242 PDK4 () primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.

Anti-PDK4 antibody - ab89295 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody (ab89295) at 1/500 dilution + Human fetal skeletal muscle lysate

Anti-PDK4 antibody - ab63157 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody (ab63157) at 1/1000 dilution + CEM cell line lysates at 35 µgSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution

Anti-PDK4 antibody - ab71240 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody (ab71240) at 1/60 dilution + HL60 cell line lysates at 35 µg

Anti-PDK4 antibody - ab88063 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody (ab88063) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PDK4 antibody - ab56020 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDK4 antibody [1C2BG5] - ab110336 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C2BG5
ICC/IF image of ab110336 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PDK4 antibody - Aminoterminal end - ab56019 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (N-Terminus) IHC-plus™ - LS-B992 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B992 concentration 5 ug/ml.

Anti-PDK4 Antibody - LS-C332347 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human colon tissue using PDK4 antibody.

Anti-PDK4 Antibody (aa382-410) IHC-plus™ - LS-B3459 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-PDK4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3459 concentration 10 ug/ml.

Anti-PDK4 Antibody (N-Terminus) - LS-C46852 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (N-Terminus) - LS-C47013 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, Biotin) - LS-C142568 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, DY650) - LS-C142569 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, DY488) - LS-C142570 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, DY550) - LS-C142572 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, HRP) - LS-C142571 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, Biotin) - LS-C142574 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, DY488) - LS-C142576 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDK4 Antibody (Internal, DY650) - LS-C142575 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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