ICC/IF image of ab110336 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-PDK4 antibody [1C2BG5] (ab110336) at 1 µg/mlLane 1 : HepG2 whole cell lysate at 10 µgLane 2 : Human heart tissue at 10 µgLane 3 : Recombinant Human PDK1 at 0.01 µgLane 4 : Recombinant Human PDK2 at 0.01 µgLane 5 : Recombinant Human PDK3 at 0.01 µgLane 6 : Recombinant Human PDK4 at 0.01 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/9363_PDK4-Primary-antibodies-ab110336-1.jpg)
All lanes : Anti-PDK4 antibody [1C2BG5] (ab110336) at 1 µg/mlLane 1 : HepG2 whole cell lysate at 10 µgLane 2 : Human heart tissue at 10 µgLane 3 : Recombinant Human PDK1 at 0.01 µgLane 4 : Recombinant Human PDK2 at 0.01 µgLane 5 : Recombinant Human PDK3 at 0.01 µgLane 6 : Recombinant Human PDK4 at 0.01 µg
![Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/9364_PDK4-Primary-antibodies-ab110336-2.jpg)
Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.