Anti-PDX1 antibody [EPR3358(2)]

• Supplier: Abcam
• Catalog: ab134150
• Prices: $388.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3358(2)
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues on the C-terminal in Human PDX1 (P52945)
• Description: Rabbit Monoclonal
• Gene: PDX1
• Conjugate: Unconjugated

Product images

ab134150 staining PDX1 in the BXPC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/150). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab134150 staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-PDX1 antibody [EPR3358(2)] (ab134150) at 1/1000 dilution + Caco-2 Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-PDX1 antibody [EPR3358(2)] (ab134150) at 1/1000 dilution + BxPC-3 Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

All lanes : Anti-PDX1 antibody [EPR3358(2)] (ab134150) at 1/1000 dilution (unpurified)Lane 1 : CACO-2 cell lysatesLane 2 : BxPC-3 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelled with ab134150, unpurified, at 1/1000 dilution.

Immunohistochemical analysis of paraffin embedded Human duodenum tissue labelled with ab134150, unpurified, at 1/1000 dilution.

ab134150, unpurified, staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/1000 in 2% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134150, unpurified, showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Heart muscles tissue using ab134150, unpurified, showing -ve staining.

Immunohistochemical analysis of paraffin embedded normal Human Normal brain tissue using ab134150, unpurified, showing -ve staining.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Rare somatic cells from human breast tissue exhibit extensive lineage plasticity. - Rare somatic cells from human breast tissue exhibit extensive lineage plasticity.

Roy S, Gascard P, Dumont N, Zhao J, Pan D, Petrie S, Margeta M, Tlsty TD. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4598-603. doi: