PDX1

Gene Symbol PDX1
Entrez Gene 3651
Alt Symbol GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1
Species Human
Gene Type protein-coding
Description pancreatic and duodenal homeobox 1
Other Description IPF-1|IUF-1|glucose-sensitive factor|insulin promoter factor 1, homeodomain transcription factor|insulin upstream factor 1|islet/duodenum homeobox-1|pancreas/duodenum homeobox protein 1|pancreatic-duodenal homeobox factor 1|somatostatin transcription factor 1|somatostatin-transactivating factor 1
Swissprots Q5VYW2 O60594 P52945
Accessions AAB47101 AAB88463 EAX08420 P52945 AF049893 AAC05157 BC111592 AAI11593 CA771381 CA848737 U30329 AAA74012 U35632 AAA88820 X99894 CAA68169 XR_941578 XR_941579 XR_941580 NM_000209 NP_000200
Function Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
Subcellular Location Nucleus. Cytoplasm, cytosol {ECO:0000250}.
Tissue Specificity Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
Top Pathways Type II diabetes mellitus, Maturity onset diabetes of the young

Pdx1 (D59H3) XP ® Rabbit mAb - 5679 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D59H3
Western blot analysis of extracts from INS-1, NIT-1 and beta-TC-6 cells using Pdx1 (D59H3) XP ® Rabbit mAb.

Pdx1 Antibody - 2437 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PDX-1 (A-17) - sc-14664 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDX-1 (A-17): sc-14664. Western blot analysis of human recombinant PDX-1 fusion protein.

PDX-1 (B-11) - sc-390792 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDX-1 (B-11): sc-390792. Western blot analysis of PDX-1 expression in non-transfected: sc-117752 (A) and human PDX-1 transfected: sc-129445 (B) 293T whole cell lysates.

PDX-1 (E-5) - sc-390808 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDX-1 (E-5): sc-390808. Western blot analysis of PDX-1 expression in non-transfected: sc-117752 (A) and human PDX-1 transfected: sc-129445 (B) 293T whole cell lysates.

PDX-1 (E-8) - sc-398502 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDX-1 (E-8): sc-398502. Western blot analysis of PDX-1 expression in non-transfected: sc-117752 (A) and human PDX-1 transfected: sc-129445 (B) 293T whole cell lysates.

PDX-1 (H-140) - sc-25403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PDX-1 (H-140): sc-25403. Western blot analysis of PDX-1 expression in non-transfected: sc-117752 (A) and human PDX-1 transfected: sc-129445 (B) 293T whole cell lysates.

PDX-1 (K-16) - sc-31967 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PDX-1 (N-18) - sc-14662 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDX-1 (N-18): sc-14662. Western blot analysis of human recombinant PDX-1 fusion protein.

Anti-PDX1 antibody - ab47383 from Abcam

Prices $395.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
Immunofluorescent staining of mouse islets using goat polyclonal anti-PDX1 antibody ab47383 (blue) and an anti-insulin antibody (green).

Anti-PDX1 antibody - ab47308 from Abcam

Prices $392.00
Sizes 100 µl
Clonality Polyclonal
Immunostaining of islets from 4 week-old mice with ab47308 guinea pig anti-PDX1 (red) and an anti-insulin antibody (green).

Anti-PDX1 antibody - ab19385 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit polyclonal antibody recognizing PDX1 (ab19385) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-PDX1 antibody - ab98298 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab98298 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-PDX1 antibody - ab79388 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDX1 antibody (ab79388) at 1/500 dilutionLane 1 : Extracts from HT-29 cellsLane 2 : Extracts from HT-29 cells with immunizing peptide at 5 µgLysates/proteins at 10 µg per lane.

Anti-PDX1 antibody [2A12] - ab84987 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A12
ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.

Anti-PDX1 antibody [EPR3358(2)] - ab134150 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3358(2)
ab134150 staining PDX1 in the BXPC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/150). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

Anti-PDX1 antibody [EPR3358(2)] (Alexa Fluor® 647) - ab196195 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3358(2)
ab196195 staining PDX1 in panc1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196195 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-PDX1 antibody - C-terminal - ab72324 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PDX1 antibody - C-terminal (ab72324) at 1/60 dilution + HepG2 cell line lysate at 35 µg

Anti-PDX1 Antibody (Thr11, AP) - LS-C280687 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDX1 Antibody (Thr11, APC) - LS-C280688 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDX1 Antibody (aa27-76) IHC-plus™ - LS-B9244 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PDX1 / IPF1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-PDX1 Antibody (Thr11, Biotin) - LS-C280689 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDX1 Antibody (Thr11, FITC) - LS-C280690 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDX1 Antibody (aa40-69) - LS-C99422 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human testis tissue reacted with PDX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PDX1 Antibody (Thr11, Azide-free, HRP) - LS-C280691 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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