All lanes : Anti-PEG10 antibody (ab151782) at 1 µg/ml (Milk blocking - 1%)Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 5 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.