Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PEG10 with ab181249 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-PEG10 antibody [EPR8156(2)] (ab181249) at 1/2000 dilutionLane 1 : JAR cell lysateLane 2 : 293 cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution