Anti-PEN2 antibody
| Name | Anti-PEN2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18189 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PEN2 |
| Blocking Peptide | Human PEN2 peptide |
| Description | Rabbit Polyclonal |
| Gene | PSENEN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PEN2 antibody (ab18189) at 2 µg/mlLane 1 : Mouse Lung LysateLane 2 : Mouse Lung Lysate with Human PEN2 peptide (ab19147) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab18189 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18189, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Presenilins promote the cellular uptake of copper and zinc and maintain copper - Presenilins promote the cellular uptake of copper and zinc and maintain copper
Greenough MA, Volitakis I, Li QX, Laughton K, Evin G, Ho M, Dalziel AH, Camakaris J, Bush AI. J Biol Chem. 2011 Mar 18;286(11):9776-86.
Characterization of an atypical gamma-secretase complex from hematopoietic - Characterization of an atypical gamma-secretase complex from hematopoietic
Placanica L, Chien JW, Li YM. Biochemistry. 2010 Apr 6;49(13):2796-804.
SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene - SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene
Donmez G, Wang D, Cohen DE, Guarente L. Cell. 2010 Jul 23;142(2):320-32.