All lanes : Anti-PEPD antibody [EPR16959] (ab197890) at 1/10000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PEPD antibody [EPR16959] (ab197890) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal placenta lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution