PEPD

Gene Symbol PEPD
Entrez Gene 5184
Alt Symbol PROLIDASE
Species Human
Gene Type protein-coding
Description peptidase D
Other Description X-Pro dipeptidase|aminoacyl-L-proline hydrolase|imidodipeptidase|proline dipeptidase|xaa-Pro dipeptidase
Swissprots A8K696 Q9BT75 A8K3Z1 P12955 B4DGJ1 A8MX47 A8K416 Q8TBN9 E9PCE8 B4DDB7
Accessions P12955 AK057538 AK225037 AK290756 BAF83445 AK290781 BAF83470 AK291561 BAF84250 AK293126 BAG56678 AK294619 BAG57802 AW512991 BC004305 AAH04305 BC015027 AAH15027 BC028295 AAH28295 BT006692 AAP35338 CR541669 CAG46470 DC385464 DC395475 DQ891114 ABM82040 DQ894294 ABM85220 J04605 AAA60064 NM_000285 NP_000276 NM_001166056 NP_001159528 NM_001166057 NP_001159529
Function Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

PEPD (A-3) - sc-390042 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PEPD (A-3): sc-390042. Western blot analysis of PEPD expression in Hep G2 whole cell lysate.

PEPD (C-18) - sc-168929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PEPD (H-205) - sc-292938 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PEPD (H-205): sc-292938. Western blot analysis of PEPD expression in non-transfected: sc-117752 (A) and human PEPD transfected: sc-113886 (B) 293T whole cell lysates.

PEPD (N-12) - sc-168931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PEPD (N-12): sc-168931. Western blot analysis of PEPD expression in U-251-MG whole cell lysate (A) and mouse placenta tissue extract (B).

PEPD (47-Q) - sc-100708 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PEPD (47-Q): sc-100708. Western blot analysis of PEPD expression in non-transfected: sc-110760 (A) and human PEPD transfected: sc-112212 (B) 293 whole cell lysates.

Anti-PEPD antibody - ab111851 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111851 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111851 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PEPD antibody - ab186851 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PEPD antibody (ab186851) at 1/500 dilutionLane 1 : BT474 cell extractLane 2 : A549 cell extractLane 3 : 293T cell extractLane 4 : SH-SY5Y cell extractLane 5 : M21 cell extract

Anti-PEPD antibody - ab86507 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PEPD antibody (ab86507) at 1 µg/ml + human placenta lysate at 10 µgSecondaryanti-Rabbit IgG HRP at 1/50000 dilution

Anti-PEPD antibody - ab55605 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PEPD antibody [5D5] - ab119016 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D5
All lanes : Anti-PEPD antibody [5D5] (ab119016) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PEPD cDNALysates/proteins at 5 µg per lane.

Anti-PEPD antibody [EPR16959] - ab197890 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16959
All lanes : Anti-PEPD antibody [EPR16959] (ab197890) at 1/10000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PEPD / PROLIDASE Antibody (aa247-493) - LS-C311837 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PEPD / PROLIDASE Antibody - LS-C341614 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PEPD / PROLIDASE Antibody (aa1-494, Azide-free) - LS-C328184 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PEPD / PROLIDASE Antibody (aa231-280) - LS-C145869 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PEPD antibody LS-C145869 Western blot of Small Intestine lysate. Antibody concentration 1 ug/ml.

Anti-PEPD / PROLIDASE Antibody (aa359-408) - LS-C102698 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
PEPD antibody LS-C102698 Western blot of Placenta lysate.

Anti-PEPD / PROLIDASE Antibody - LS-C334052 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PEPD antibody.

Anti-PEPD / PROLIDASE Antibody (aa22-290) - LS-C178593 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PEPD / PROLIDASE Antibody (clone 1B7) IHC-plus™ - LS-B11014 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B7
Anti-PEPD / PROLIDASE antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11014 concentration 10 ug/ml.

Anti-PEPD / PROLIDASE Antibody (clone 1D5-H3) - LS-C197874 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D5-H3
Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 ug/ml]

PEPD Antibody (1D5-H3) - H00005184-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D5-H3
Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - PEPD monoclonal antibody (M01), clone 1D5-H3 Analysis of PEPD expression in HepG2.

PEPD Antibody - NBP1-86072 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PEPD Antibody [NBP1-86072] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp.  Lane 4: Human plasma (IgG/HSA depleted).  Lane 5: Human liver tissue.  Lane 6: Human tonsil tissue

PEPD Antibody - NBP1-56815 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PEPD Antibody [NBP1-56815] - Human Placenta lysate, concentration 0.2-1 ug/ml.

PEPD Antibody - H00005184-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PEPD Antibody [H00005184-D01P] - PEPD MaxPab rabbit polyclonal antibody. Western Blot analysis of PEPD expression in human kidney.

PEPD Antibody - H00005184-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PEPD Antibody [H00005184-B01] - PEPD MaxPab polyclonal antibody (B01), Lot # 08043 WULz. Western Blot analysis of PEPD expression in human placenta.
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