Anti-Perforin antibody [B-D48]

• Supplier: Abcam
• Catalog: ab47225
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: B-D48
• Applications: WB FC IHC-F
• Species Reactivities: Human
• Antigen: Recombinant human perforin
• Description: Mouse Monoclonal
• Gene: PRF1
• Conjugate: Unconjugated

Product images

IHC image of Perforin staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47225, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule - Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule

Liu S, Zhang H, Li M, Hu D, Li C, Ge B, Jin B, Fan Z. Cell Death Differ. 2013 Mar;20(3):456-64.

The PP2A inhibitor SET regulates granzyme B expression in human natural killer - The PP2A inhibitor SET regulates granzyme B expression in human natural killer

Trotta R, Ciarlariello D, Dal Col J, Mao H, Chen L, Briercheck E, Yu J, Zhang J, Perrotti D, Caligiuri MA. Blood. 2011 Feb 24;117(8):2378-84.

Enrichment and analysis of secretory lysosomes from lymphocyte populations. - Enrichment and analysis of secretory lysosomes from lymphocyte populations.

Schmidt H, Gelhaus C, Lucius R, Nebendahl M, Leippe M, Janssen O. BMC Immunol. 2009 Jul 29;10:41.