Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
| Name | Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab28144 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mAbcam 28144 |
| Applications | ICC/IF ICC/IF FC |
| Species Reactivities | Human, Hamster |
| Antigen | The pericentrin clone used is 1 |
| Description | Mouse Monoclonal |
| Gene | PCNT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28144 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28144, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
![Overlay histogram showing HeLa cells stained with ab28144 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28144, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/9882_Pericentrin-Primary-antibodies-ab28144-7.jpg)
Overlay histogram showing HeLa cells stained with ab28144 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28144, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 - Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1
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Pericentrosomal localization of the TIG3 tumor suppressor requires an N-terminal - Pericentrosomal localization of the TIG3 tumor suppressor requires an N-terminal
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TIG3 tumor suppressor-dependent organelle redistribution and apoptosis in skin - TIG3 tumor suppressor-dependent organelle redistribution and apoptosis in skin
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