PCNT

Gene Symbol PCNT
Entrez Gene 5116
Alt Symbol KEN, MOPD2, PCN, PCNT2, PCNTB, PCTN2, SCKL4
Species Human
Gene Type protein-coding
Description pericentrin
Other Description kendrin|pericentrin-2|pericentrin-380|pericentrin-B
Swissprots O43152 Q7Z7C9 O95613
Accessions CBX47507 EAX09277 EAX09278 EAX09279 EAX09280 EAX09281 O95613 AB007862 BAA23698 AF515282 AAP46636 AK024009 BAB14772 AK093923 BAC04252 AK296731 BAG59318 AW452809 BC035913 AAH35913 BP311784 BP312870 DC345793 U52962 AAD10838 XM_005261124 XP_005261181 XM_005261129 XP_005261186 XM_011529593 XP_011527895 XM_011529594 XP_011527896 NM_006031 NP_006022
Function Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. {ECO:0000269|PubMed:10823944, ECO:0000269|PubMed:11171385, ECO:0000269|PubMed:18955030, ECO:0000269|PubMed:20599736}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:10823944, ECO:0000269|PubMed:11171385, ECO:0000269|PubMed:18955030, ECO:0000269|PubMed:22797915}. Note=Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
Tissue Specificity Expressed in all tissues tested, including placenta, liver, kidney and thymus. {ECO:0000269|PubMed:10823944}.

Pericentrin 2 (H-140) - sc-68928 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pericentrin 2 (H-140): sc-68928. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.

Pericentrin 2 (C-16) - sc-28145 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pericentrin 2 (C-16): sc-28145. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.

Pericentrin 2 (N-20) - sc-28143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pericentrin 2 (S-20) - sc-28144 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Pericentrin antibody - ab99341 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

Anti-Pericentrin antibody - ab84542 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84542 at 1/100 dilution staining Pericentrin in HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.

Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker - ab28144 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 28144
ICC/IF image of ab28144 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28144, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-Pericentrin antibody - Centrosome Marker - ab4448 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab4448 stained NIH-3T3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PCNT / Pericentrin Antibody - LS-C183405 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PCNT / Pericentrin Antibody (aa3286-3336) - LS-C288157 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PCNT / Pericentrin Antibody - LS-C342157 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PCNT / Pericentrin Antibody (aa175-225) - LS-C288158 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Pericentrin/Kendrin by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1000 (1 ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4) used at a dilution of 1:100.

Anti-PCNT / Pericentrin Antibody (aa175-225) - LS-C286509 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Pericentrin/Kendrin by Immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:500. Detection: Red-fluorescent Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen).

Pericentrin Antibody - NB100-61071 from Novus Biologicals

Prices $359.00, $37,100.00
Sizes 100 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Acetone-fixed asynchronous HeLa cells. Affinity purified rabbit anti- Pericentrin/Kendrin used at a dilution of 1:100.

Pericentrin Antibody - NBP1-87771 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Pericentrin Antibody - NBP1-87772 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PCNT Polyclonal Antibody - bs-7711R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PCNT antibody produced in rabbit - HPA019887 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PCNT: <B>Cat. No. HPA019887</B>: Immunohistochemical staining of human placenta shows distinct dot-like positivity in decidual cells.

Anti-PCNT antibody produced in rabbit - HPA016820 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PCNT: <B>Cat. No. HPA016820</B>: Immunohistochemical staining of human exocrine pancreas shows spot-like perinuclear positivity.

Pericentrin antibody - GTX51815 from GeneTex

Prices $319.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal

PCNT antibody - orb100238 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin embedded mouse embryo tissue using PCNT antibody (dilution at 1:200)

Pericentrin Antibody - abx007839 from Abbexa

Host Rabbit
Clonality Polyclonal

Anti-Pericentrin Antibody - 923701 from Biolegend

Prices $320.00
Sizes 200 µl
Host Rabbit
Clone Poly19237

Pericentrin antibody - 22271-1-AP from Proteintech Group

Prices $279.00
Sizes con: 54 μg/150μl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed MDCK cells using 22271-1-AP( Pericentrin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Pericentrin/Kendrin Antibody - A301-348A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (1 mg/ml)
Host Rabbit
Clonality Polyclonal
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