Anti-Periostin antibody

• Supplier: Abcam
• Catalog: ab14041
• Prices: $403.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F IHC-P WB ELISA
• Species Reactivities: Mouse, Rat, Chicken, Human
• Antigen: Recombinant fusion protein (Human) produced in E
• Description: Rabbit Polyclonal
• Gene: POSTN
• Conjugate: Unconjugated

Product images

ab14041 staining Periostin in human differentiated cardiac stem cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/800 in permeabilization buffer) for 1 hour at 4°C. ab175651, an Alexa Fluor® 405-conjugated donkey polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.Gating Strategy: Isotype negative control (white). See Abreview

ab14041 at a 1/100 dilution staining Periostin in mouse dermis tissue by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized using 0.1% Triton. Blocked using 20% serum for 1 hour at 4°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.See Abreview

ICC/IF image of ab14041 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab14041, staining Periostin in Human breast carcinoma tissue by Immunohistochemistry (Frozen sections). Sections were acetone-fixed and blocked in normal serum. The sections were incubated with the primary antibody (for 1 hour at room temperature), followed by incubation with an HRP-conjugated secondary antibody for additional 30 minutes at room temperature. Diaminobenzidine (DAB) was used as a chromogen resulting in brown staining of positive cells. The nuclei were counterstained in using hematoxylin.

Product References

Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant - Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant

Thorfve A, Lindahl C, Xia W, Igawa K, Lindahl A, Thomsen P, Palmquist A, Tengvall P. Acta Biomater. 2014 Mar;10(3):1451-62.

Astroglial-derived periostin promotes axonal regeneration after spinal cord - Astroglial-derived periostin promotes axonal regeneration after spinal cord

Shih CH, Lacagnina M, Leuer-Bisciotti K, Proschel C. J Neurosci. 2014 Feb 12;34(7):2438-43.

The impact of flow-induced forces on the morphogenesis of the outflow tract. - The impact of flow-induced forces on the morphogenesis of the outflow tract.

Biechler SV, Junor L, Evans AN, Eberth JF, Price RL, Potts JD, Yost MJ, Goodwin RL. Front Physiol. 2014 Jun 17;5:225.

Gene expression profiling of peri-implant healing of PLGA-Li+ implants suggests - Gene expression profiling of peri-implant healing of PLGA-Li+ implants suggests

Thorfve A, Bergstrand A, Ekstrom K, Lindahl A, Thomsen P, Larsson A, Tengvall P. PLoS One. 2014 Jul 21;9(7):e102597.

Periostin cooperates with mutant p53 to mediate invasion through the induction of - Periostin cooperates with mutant p53 to mediate invasion through the induction of

Wong GS, Lee JS, Park YY, Klein-Szanto AJ, Waldron TJ, Cukierman E, Herlyn M, Gimotty P, Nakagawa H, Rustgi AK. Oncogenesis. 2013 Aug 5;2:e59.

The matricellular protein periostin contributes to proper collagen function and - The matricellular protein periostin contributes to proper collagen function and

Egbert M, Ruetze M, Sattler M, Wenck H, Gallinat S, Lucius R, Weise JM. J Dermatol Sci. 2014 Jan;73(1):40-8.

The AP-1 transcription factor c-Jun prevents stress-imposed maladaptive - The AP-1 transcription factor c-Jun prevents stress-imposed maladaptive

Windak R, Muller J, Felley A, Akhmedov A, Wagner EF, Pedrazzini T, Sumara G, Ricci R. PLoS One. 2013 Sep 10;8(9):e73294.

Gene expression profiling-derived immunohistochemistry signature with high - Gene expression profiling-derived immunohistochemistry signature with high

Chang W, Gao X, Han Y, Du Y, Liu Q, Wang L, Tan X, Zhang Q, Liu Y, Zhu Y, Yu Y, Fan X, Zhang H, Zhou W, Wang J, Fu C, Cao G. Gut. 2014 Sep;63(9):1457-67.

Periostin inhibits hypoxia-induced apoptosis in human periodontal ligament cells - Periostin inhibits hypoxia-induced apoptosis in human periodontal ligament cells

Aukkarasongsup P, Haruyama N, Matsumoto T, Shiga M, Moriyama K. Biochem Biophys Res Commun. 2013 Nov 8;441(1):126-32. doi:

Overexpression of periostin predicts poor prognosis in non-small cell lung - Overexpression of periostin predicts poor prognosis in non-small cell lung

Hong LZ, Wei XW, Chen JF, Shi Y. Oncol Lett. 2013 Dec;6(6):1595-1603. Epub 2013 Sep 18.