Anti-Periostin antibody
| Name | Anti-Periostin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92460 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Chicken, Human, Bird, Fish, Mammalian, Amphibians |
| Antigen | Synthetic peptide from the fasciclin domain 1 of mouse periostin |
| Description | Rabbit Polyclonal |
| Gene | POSTN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Periostin antibody (ab92460) at 1/1000 dilution + rat lung lysate
ICC/IF image of ab92460 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab92460 staining in Hu Aorta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92460, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Nifedipine induces periostin expression in gingival fibroblasts through TGF-beta. - Nifedipine induces periostin expression in gingival fibroblasts through TGF-beta.
Kim SS, Jackson-Boeters L, Darling MR, Rieder MJ, Hamilton DW. J Dent Res. 2013 Nov;92(11):1022-8.
Epicardial function of canonical Wnt-, Hedgehog-, Fgfr1/2-, and - Epicardial function of canonical Wnt-, Hedgehog-, Fgfr1/2-, and
Rudat C, Norden J, Taketo MM, Kispert A. Cardiovasc Res. 2013 Dec 1;100(3):411-21.