Anti-Periostin antibody (ab83739) at 1 µg/ml + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ab83690 at 5 µg/ml staining Periostin in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
ab83690 at 5 µg/ml staining periostin in Human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
IHC image of ab83739 staining in Hu Aorta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83739, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.