Anti-Periplakin antibody
| Name | Anti-Periplakin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72422 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Rat, Human, Guinea Pig, Bovine, Xenopus, Chimpanzee, Amphibians, Monkey, Ape, Orangutan, Fish |
| Antigen | Synthetic peptide corresponding to a region between residues 1150 and 1200 of human Periplakin (NP_002696 |
| Description | Rabbit Polyclonal |
| Gene | PPL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab72422 stained SW480 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72422 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-Periplakin antibody (ab72422) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
Detection of Periplakin by Western Blot of Immunprecipitate. ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.