Anti-Peroxiredoxin 2 antibody
| Name | Anti-Peroxiredoxin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15572 |
| Prices | $394.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ICC/IF |
| Species Reactivities | Human, Monkey |
| Antigen | Synthetic peptide corresponding to Human Peroxiredoxin 2 aa 104-115 |
| Description | Rabbit Polyclonal |
| Gene | PRDX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Staining of Peroxiredoxin 2 in TSU PR1 cells using ab15572.
ICC/IF image of ab15572 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15572, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab15572 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15572, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Site-specific mapping and quantification of protein S-sulphenylation in cells. - Site-specific mapping and quantification of protein S-sulphenylation in cells.
Yang J, Gupta V, Carroll KS, Liebler DC. Nat Commun. 2014 Sep 1;5:4776.
Circadian clocks in human red blood cells. - Circadian clocks in human red blood cells.
O'Neill JS, Reddy AB. Nature. 2011 Jan 27;469(7331):498-503.