Anti-Peroxiredoxin 2 antibody [1E8]
| Name | Anti-Peroxiredoxin 2 antibody [1E8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab50862 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1E8 |
| Applications | ELISA WB FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant human protein purified from E |
| Description | Mouse Monoclonal |
| Gene | PRDX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Peroxiredoxin 2 antibody [1E8] (ab50862) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HepG2 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10032_ab50862.jpg)
All lanes : Anti-Peroxiredoxin 2 antibody [1E8] (ab50862) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HepG2 cell lysate
![Overlay histogram showing HeLa cells stained with ab50862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56802, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10033_Peroxiredoxin-2-Primary-antibodies-ab50862-1.jpg)
Overlay histogram showing HeLa cells stained with ab50862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56802, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Peroxiredoxin post-translational modifications by redox messengers. - Peroxiredoxin post-translational modifications by redox messengers.
Riquier S, Breton J, Abbas K, Cornu D, Bouton C, Drapier JC. Redox Biol. 2014 Jun 5;2:777-85.
p10, the N-terminal domain of p35, protects against CDK5/p25-induced - p10, the N-terminal domain of p35, protects against CDK5/p25-induced
Zhang L, Liu W, Szumlinski KK, Lew J. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):20041-6. doi:
Peroxiredoxin 2 and peroxidase enzymatic activity of mammalian spermatozoa. - Peroxiredoxin 2 and peroxidase enzymatic activity of mammalian spermatozoa.
Manandhar G, Miranda-Vizuete A, Pedrajas JR, Krause WJ, Zimmerman S, Sutovsky M, Sutovsky P. Biol Reprod. 2009 Jun;80(6):1168-77.