Anti-Peroxiredoxin 2 antibody [EPR5154]

• Supplier: Abcam
• Catalog: ab109367
• Prices: $398.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5154
• Applications: FC WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Peroxiredoxin 2 aa 1-100 (internal sequence)
• Description: Rabbit Monoclonal
• Gene: PRDX2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab109367 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109367, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] (ab109367) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : LnCaP cell lysateLane 4 : SH-SYSY cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Immunofluorescent staining of Peroxiredoxin 2 in HeLa cells using ab109367 at 1/250 dilution.

Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human prostatic hyperplasia tissue using ab109367 at 1/1000 dilution.

Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human stomach tissue using ab109367 at 1/1000 dilution.

ab109367 (1/500) staining Peroxiredoxin 2 in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview

Product References

Deficits in receptor-mediated endocytosis and recycling in cells from mice with - Deficits in receptor-mediated endocytosis and recycling in cells from mice with

Zhou GL, Na SY, Niedra R, Seed B. J Cell Sci. 2014 Sep 15;127(Pt 18):3916-27.