Anti-Pescadillo antibody (ab88543) at 1 µg/ml + PC-12 cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Anti-Pescadillo antibody (ab88543) at 1 µg/ml + A-431 cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
All lanes : Anti-Pescadillo antibody (ab88543) at 1 µg/mlLane 1 : Pescadillo transfected 293T cell lysate Lane 2 : Non transfected 293T cell lysate Lysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ab88543, at 10 µg/ml, staining Pescadillo in HeLa cell by Immunofluorescence.