Anti-PGC1 alpha + beta antibody
| Name | Anti-PGC1 alpha + beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72230 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine, Dog, Pig, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human PGC1 alpha |
| Description | Rabbit Polyclonal |
| Gene | PPARGC1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. -ve control 2 is ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mlLane 1 : Testis (Mouse) Tissue LysateLane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 3 : Ovary (Rat) Tissue LysateLane 4 : Colon (Mouse) Tissue LysateLane 5 : Skeletal Muscle (Mouse) Tissue LysateLane 6 : Thymus (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Colon (Mouse) Tissue LysateLane 3 : Thymus (Rat) Tissue LysateLane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) with Human PGC1 alpha peptide (ab73600) at 1 µg/mlLane 5 : Colon (Mouse) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/mlLane 6 : Thymus (Rat) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/mlLane 7 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) with PGC1 beta peptide at 1 µg/mlLane 8 : Colon (Mouse) Tissue Lysate with PGC1 beta peptide at 1 µg/mlLane 9 : Thymus (Rat) Tissue Lysate with PGC1 beta peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.Ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.Orange line: ELISA against immunising peptide ab73600Yellow line: ELISA against ab88232
Product References
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